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Image Search Results
Journal: Molecular and Cellular Biology
Article Title: Defective Flux of Thrombospondin-4 through the Secretory Pathway Impairs Cardiomyocyte Membrane Stability and Causes Cardiomyopathy
doi: 10.1128/MCB.00114-18
Figure Lengend Snippet: Impaired sarcolemmal glycoprotein trafficking in hearts overexpressing secretion-defective Thbs4. (A) Western blotting for membrane glycoproteins purified from hearts of transgenic mice overexpressing wild-type Thbs4 (DTG-Thbs4) or the secretion-defective mutant (DTG-mCa2+). Cadherin was used as a loading control. (B) Representative experiment of immunocytochemistry for β-sarcoglycan and the Golgi marker GM130 in cardiomyocytes isolated from transgenic hearts of the indicated genotype. Scale bar = 50 μm. Similar results were obtained in 2 additional experiments.
Article Snippet: Proteins were resolved by SDS-PAGE, transferred to polyvinylidene difluoride (PVDF) membranes (Millipore), and immunoblotted with antibodies for Flag (Cell Signaling Technology or Sigma), calreticulin, PDI, integrin β1, pan-cadherin, and Rab6 (Cell Signaling Technology), Armet, SAR1, δ-sarcoglycan, and β-actin (Abcam),
Techniques: Western Blot, Membrane, Purification, Transgenic Assay, Mutagenesis, Control, Immunocytochemistry, Marker, Isolation